Most buffers are made through titration or Henderson Hasselbach. However, here is a non-exhaustive list of the buffers that I have found of use.

50X TAE (Tris/Acetic Acid/EDTA)

1. 242g Tris-base
2. 100mL 0.5M EDTA
3. 57.1mL glacial acetic acid
4. Add diH2O to 1L
5. To make 1x TAE, dilute 20mL. Ex: 10L carboy multiply by 10 to get 200mL of TAE stock.

Alternatively can use pre-made Bio-Rad TAE stock (1 L #1610743). Measure 10mL of stock, add 490mL of diH2O, and mix vigorously.

10X Laemmli Electrophoresis Running Buffer

http://cshprotocols.cshlp.org/content/2006/5/pdb.rec10664.full?text_only=true

1. Dissolve the following components in 1000 mL MilliQ
   1. 30.0g Tris base
   2. 144.0g glycine
   3. 10.0g SDS
2. The pH of the buffer should be 8.3 and no pH adjustment is required. Store the running buffer at room temperature and dilute to 1x before use.

Bacterial Resuspension Buffer

Developed in Bouyain Lab. Buffer can be modified to end user’s desire. Makes 1L.

Final concentration: 0.5M NaCl, 50mM Tris pH 8.0, 5% (w/v) glycerol, 10 mM Imidazole pH 8.0.

Rationale: Higher amounts of sodium chloride can keep bacterially expressed proteins soluble. Can be lowered to 300mM. Glycerol can prevent aggregation and improve solubility. Imidazole can prevent non-specific binding to IMAC resin.