Processing structural biology data is challenging. Whether dealing with experimental NMR, crystallography, or cryo-EM data one only becomes “proficient” when processing their own datasets.

csparc2star.py

Choosing an Extraction Box Size

This diagram shows the standard preprocessing workflow for cryo-EM data, starting from raw data collection through 2D classification. Each step represents a crucial phase in data preparation.


Preprocessing

Steps for preprocessing data in both cryoSPARC or RELION.

cryoSPARC

cryoSPARC Pre-Processing

RELION

RELION Pre-Processing